SHANK2 mouse models show opposite brain signaling
Two new strains of mice carrying different mutations in the SHANK2 gene show similar autism-like behaviors but opposing effects on brain signaling, according to two independent studies published 14 June in Nature1,2.
One of the strains, developed by Korean researchers, has lower-than-normal activity at so-called NMDA receptors, triggered by the neurotransmitter glutamate. Drugs that boost NMDA signaling restore these animals’ social behaviors but not their repetitive behaviors or hyperactivity, the researchers found.
The findings highlight how different mutations in the same gene can lead to profoundly different molecular effects, making it challenging for drug makers to develop treatments that will be effective across the diverse autism spectrum.
“The mice are very opposite in some measures of synaptic function, and that’s very interesting,” says Ozlem Bozdagi-Gunal, assistant professor of psychiatry at Mount Sinai School of Medicine in New York City, who was not involved in either study.
It will take a long time, Bozdagi-Gunal adds, to understand how these molecular differences lead to the same behaviors in mice, and how they contribute to autism in people. Unlike mouse models of fragile X syndrome, which have relatively well-characterized molecular underpinnings, “in SHANK models, the interactions are more complicated,” she says.
The SHANK2 mouse developed by Boeckers debuted last year at the Society for Neuroscience annual meeting, and carries a mutation in exon 7. (An exon is one piece of a protein-coding section of the genome.) In contrast, mice in the Korean study lack exons 6 and 7 — the same regions missing in individuals with autism.
|Popcorn mice: SHANK2 mice repeatedly jump up against walls, a behavior that has not been observed in other animal models of autism.|
Because both mutations interfere with the normal workings of the SHANK2 protein, it’s unclear why they cause opposite patterns in neuron signaling, notes Min Goo Lee, professor of pharmacology at Yonsei University College of Medicine in Seoul, who helped develop the Korean SHANK2 mouse.
Given other recent results, however, it’s not surprising that subtle differences in the type of mutations can have dramatically diverse effects in the brain, says Bozdagi-Gunal.
This may be because different domains of a protein have different functions. “The binding proteins for one domain are different than another domain, and so that can make the signaling different,” she says.
The implications for drug development are similarly complex.
In the new study, the researchers reversed many of the animals’ social deficits by giving one of two drugs. The first, called D-cycloserine, was originally used as an antibiotic for treating tuberculosis. Researchers later discovered that it partially activates NMDA receptors, leading to its testing for psychiatric disorders such as schizophrenia3 and obsessive-compulsive disorder4, with promising preliminary results.
The drug’s effects in people are unclear, though a 2004 pilot study tested it in ten children and young adults with autism and found that it improves social interactions5. No follow-up studies have been published, however. The drug has several known side effects, including irritability, depression and convulsions.
The other drug tested in the new study is CDPPB, which indirectly boosts NMDA function by activating another glutamate receptor called mGluR5. Intriguingly, mGluR5 is overactive in mouse models of fragile X syndrome, and several clinical trials are testing mGluR5 blockers to treat fragile X syndrome and autism.
That both mGluR5 activators and blockers seem to alleviate symptoms of autism in different animal models “is particularly perplexing,” says Craig Powell, professor of neurology and neurotherapeutics at the University of Texas Southwestern in Dallas. He points out, though, that some forms of autism stem from either a deletion or a duplication of the same gene. Another study, published late last year, found opposite molecular signatures in mouse models of fragile X syndrome and tuberous sclerosis, both autism-related disorders.
“This may represent another ‘Goldilocks’ analogy where too much and too little of a good thing are deleterious,” Powell says. “In the treatment of autism, we should certainly proceed with caution and aim for ‘just right.’”
1: Won H. et al. Nature 486, 261-265 (2012) Abstract
2: Schmeisser M.J. et al. Nature 486, 256–260 (2012) Abstract
3: Gottlieb J.D. et al. Schizophr. Res. 131, 69-74 (2011) PubMed
4: Wilhelm S. et al. Am. J. Psychiatry 165, 335-341 (2008) PubMed
5: Posey D.J. et al. Am. J. Psychiatry 161, 2115-2117 (2004) PubMed